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+WORD: This avoids the coupling of amino acids within the further cycles with the [membrane](https://www.ft.com/search?q=membrane) as a substitute of the rising peptide chain. OBSERVE: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino teams between the spots and also a few of the amino teams throughout the spot areas don't type bonds with the amino acids. Be certain all of the purification buffers are at four °C and carry out the protein purification in the cold room to keep away from protein denaturation.
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+Widespread posttranslational modifications include methylation of the ε-amino group, giving methyl- , dimethyl- , and trimethyllysine The latter occurs in calmodulin Other posttranslational modifications at lysine residues embrace acetylation , sumoylation , and ubiquitination Collagen contains hydroxylysine , which is derived from lysine by lysyl hydroxylase - Glycosylation of hydroxylysine residues in the endoplasmic reticulum or Golgi equipment is used to mark sure proteins for secretion from the cell In opsins like rhodopsin and the visual opsins (encoded by the genes OPN1SW , OPN1MW , and OPN1LW ), retinaldehyde kinds a Schiff base with a conserved lysine residue, and interplay of sunshine with the retinylidene group causes sign transduction in shade imaginative and prescient (See visible cycle for particulars).
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